SR-FLICA® Poly Caspase 檢測試劑盒

廠牌: Immunochemistry 產地: 美國

SR-FLICA® Poly Caspase Assay Kit
貨號:916

售價: 9985元
Size: 25 Tests

使用 SR FLICA® Poly Caspase 檢測試劑盒檢測活性半胱天冬酶。這種體外測定採用螢光抑製劑探針 SR-VAD-FMK 來標記活細胞中的活性半胱天冬酶。使用螢光顯微鏡、螢光盤式分析儀或流式細胞儀分析樣品。


Background Caspases play important roles in apoptosis and inflammation. ICT’s FLICA poly caspase assay kit is used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.


Reagent Name SR-VAD-FMK


Target Poly caspases


Excitation / Emission 550-580 nm / 590-600 nm


Method of Analysis Flow Cytometer, Fluorescence Plate Reader, Fluorescence Microscope


Sample Type Cell culture


Storage 2-8°C


Country of Origin United States


Protocols

  1. Prepare samples and controls.

  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.

  3. Reconstitute FLICA with 50 μL DMSO.

  4. Dilute FLICA 1:5 by adding 200 μL PBS.

  5. Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells.

  6. Incubate approximately 1 hour.

  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells.

  8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody.

  9. If desired, fix or embed cells.

  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.


Kit Contents

Kit# 916: 25 Tests FLICA Poly Caspase Reagent (SR-VAD-FMK), 1 vial, #679 10X Apoptosis Wash Buffer, 15 mL, #635 Fixative, 6 mL, #636 Hoechst 33342, 1 mL, #639 Kit Manual


Citations (3)

Nougarede A, Popgeorgiev N, Kassem L, Omarjee S, Borel S, Mikaelian I, Lopez J, Gadet R, Marcillat O, Treilleux I, Villoutreix BO, Rimokh R, Gillet G. Breast cancer targeting through inhibition of the endoplasmic reticulum-based apoptosis regulator Nrh/BCL2L10. Cancer Res. 2018. Jan 12. pii: canres.0846.2017. doi: 10.1158/0008-5472.CAN-17-0846. [Epub ahead of print]. Abstract Schell SL, Soni C, Fasnacht MJ, Domeier PP, Cooper TK, Rahman ZSM. Mer Receptor Tyrosine Kinase Signaling Prevents Self-Ligand Sensing and Aberrant Selection in Germinal Centers. J. Immunol. Dec 15;199(12):4001-4015. doi: 10.4049/jimmunol.1700611. Epub 2017 Nov 8. Abstract Marschall M, Simnacher U, Walther P, Essig A, Hagemann J. The Putative Type III Secreted Chlamydia abortus Virulence-Associated Protein CAB063 Targets Lamin and Induces Apoptosis. Front Microbiol. 2020 May 25;11:1059. doi: 10.3389/fmicb.2020.01059. eCollection 2020. Full Text