Hoechst 33342 螢光核酸染色劑

Hoechst 33342 核酸染料是一種常用的細胞滲透核複染劑，當與 dsDNA 結合時會發出藍色螢光。這種染料通常用於區分凋亡細胞中的濃縮固縮核和用於細胞週期研究。

Background Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.

Excitation / Emission 350 nm / 480 nm

Method of Analysis Flow cytometry, Fluorescence microscope

Storage 2-8°C

PH 5.0 + 0.5

Supplied At 200 µg/mL

Country of Origin United States

Protocols

1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 µL Hoechst to 300 µL of cells. 2. Incubate 10-20 minutes at room temperature.

2. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.

3. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.

4. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.

Citations (9)

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