Hoechst 33342 螢光核酸染色劑

廠牌: Immunochemistry 產地: 美國

Hoechst 33342 Fluorescent Nucleic Acid Stain
貨號:639

售價: 3918元
Size: 1 mL

Hoechst 33342 核酸染料是一種常用的細胞滲透核複染劑,當與 dsDNA 結合時會發出藍色螢光。這種染料通常用於區分凋亡細胞中的濃縮固縮核和用於細胞週期研究。


Background Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.


Excitation / Emission 350 nm / 480 nm


Method of Analysis Flow cytometry, Fluorescence microscope


Storage 2-8°C


PH 5.0 + 0.5


Supplied At 200 µg/mL


Country of Origin United States


Protocols

  1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 µL Hoechst to 300 µL of cells. 2. Incubate 10-20 minutes at room temperature.

  2. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.

  3. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.

  4. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.


Citations (9)

Castelli, R;Ibarra, M;Faccio, R;Miraballes, I;Fernández, M;Moglioni, A;Cabral, P;Cerecetto, H;Glisoni, R;Calzada, V. T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures. Pharmaceuticals. 2021 December 23; doi: 10.3390/ph15010015. Article Andreeva, L;David, L;Rawson, S;Shen, C;Pasricha, T;Pelegrin, P;Wu, H. NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation. Cell. 2021 December 22; doi: 10.1016/j.cell.2021.11.011. Article Niu, T;De Rosny, C;Chautard, S;Rey, A;Patoli, D;Groslambert, M;Cosson, C;Lagrange, B;Zhang, Z;Visvikis, O;Hacot, S;Hologne, M;Walker, O;Wong, J;Wang, P;Ricci, R;Henry, T;Boyer, L;Petrilli, V;Py, BF. NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly. Nature communications. 2021 October 6; doi: 10.1038/s41467-021-26142-w. Article Brempelis KJ, Cowan CM, Kreuser SA, Labadie KP, Prieskorn BM, Lieberman NAP, Ene CI, Moyes KW, Chinn H, DeGolier KR, Matsumoto LR, Daniel SK, Yokoyama JK, Davis AD, Hoglund VJ, Smythe KS, Balcaitis SD, Jensen MC, Ellenbogen RG, Campbell JS, Pierce RH, Holland EC, Pillarisetty VG, Crane CA. Genetically engineered macrophages persist in solid tumors and locally deliver therapeutic proteins to activate immune responses. J Immunother Cancer. 2020 Oct;8(2):e001356. doi: 10.1136/jitc-2020-001356. Full Text Crespo ÂC, Mulik S, Dotiwala F, Ansara JA, Sen Santara S, Ingersoll K, Ovies C, Junqueira C, Tilburgs T, Strominger JL, Lieberman J. Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts. Cell. 2020 Sep 3;182(5):1125-1139.e18. doi: 10.1016/j.cell.2020.07.019. Epub 2020 Aug 20. Abstract Huang K, Miao T, Chang K, Kim J, Kang P, Jiang Q, Simmonds AJ, Di Cara F, Bai H. Impaired peroxisomal import in Drosophila oenocytes causes cardiac dysfunction by inducing upd3 as a peroxikine. Nat Commun. 2020 Jun 10;11(1):2943. doi: 10.1038/s41467-020-16781-w. Full Text Cho HJ, Yang EJ, Park JT, Kim JR, Kim EC, Jung KJ, Park SC, Lee YS. Identification of SYK inhibitor, R406 as a novel senolytic agent. Aging (Albany NY). 2020 May 7;12(9):8221-8240. doi: 10.18632/aging.103135. Epub 2020 May 7. Full Text Czimmerer Z, Daniel B, Horvath A, Rückerl D, Nagy G, Kiss M, Peloquin M, Budai MM, Cuaranta-Monroy I, Simandi Z, Steiner L, Nagy B, Poliska S, Banko C, Bacso Z, Schulman IG, Sauer S, Deleuze JF, Allen JE, Benko S, Nagy L. The Transcription Factor STAT6 Mediates Direct Repression of Inflammatory Enhancers and Limits Activation of Alternatively Polarized Macrophages. Immunity. 2018. Jan 16;48(1):75-90.e6. doi: 10.1016/j.immuni.2017.12.010. Full text